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KMID : 1025520040460030307
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Journal of Animal Science and Technology
2004 Volume.46 No. 3 p.307 ~ p.314
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Prevalence of PERVs from Domestic Pigs in Korea (pol gene sequences)
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Kim Young-Boong
Yoo Jae-Young
Lee J.-Y.
Kim Gye-Woong
Park Hong-Yang
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Abstract
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Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organs available for human transplantation. The swine represents an ideal source of such organs because of their plentiful supply and their numerous anatomical and physiological similarities to the human. However, this procedure also carries with a number of safety issues relating to the zoonotic infections. Porcine endogenous retroviruses(PERVs), which are germ line transmitted and persist without symptoms in the pigs, are most concerning zoonotic viruses. In order to analyze the prevalence of PERV in domestic pigs, four kinds of pigs’(Landrace, Berkshire, Yorkshire, and Duroc) genomic DNA were isolated from their hair follicles. PCR analysis was carried out for detection of PERVs using subgroup A/B/C and E pol sequence primers. All pigs (20 heads) tested had high copy number of PERVs within genomes. Subgroup A/B/C and E pol gene sequences from 20 isolates were determined by direct sequencing. Sequence analysis showed pol sequences are highly conserved among intra- and inter-subspecies(99.1 and 98.8%, respectively). As a first report of PERV prevalence in Korea pigs, our data would be the basic concepts of PERV transmission study in xenotransplantation.
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KEYWORD
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PERV, Xenotransplantation, gDNA, Pig, Korea
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